How to Reconstitute Tesamorelin: Lab Protocol with Dilution Math

Tesamorelin is a synthetic GHRH (growth hormone-releasing hormone) analogue supplied in lyophilized form for research applications. This protocol covers materials, reconstitution math for the standard vial sizes, post-reconstitution stability, and storage. Written for in-vitro and animal research models only — not a dosing recommendation.

Materials

1 vial of lyophilized tesamorelin (5mg / 10mg / 20mg)
Sterile bacteriostatic water (0.9% benzyl alcohol)
1 mL insulin syringe (U-100), 28–31G needle
Alcohol prep pads
Refrigerator (2–8°C) for storage

Why bacteriostatic water

GHRH-class peptides are sensitive to repeated handling and benefit from the preservative in bacteriostatic water (0.9% benzyl alcohol), which extends usable life of the reconstituted solution compared with plain sterile water. Plain sterile water limits the solution to ~24 hours and is not recommended for multi-day research protocols.

Reconstitution math

The volume of bacteriostatic water added sets the concentration of the reconstituted solution. The table below shows clean per-tick math on a U-100 insulin syringe for the three common vial sizes.

Vial	BAC water added	Concentration	Per 10 IU tick (0.10 mL)
5mg tesamorelin	2.0 mL	2.5 mg/mL	0.25 mg
10mg tesamorelin	2.0 mL	5 mg/mL	0.5 mg
20mg tesamorelin	2.0 mL	10 mg/mL	1.0 mg

Step-by-step procedure

Inspect the lyophilized vial. The cake should be a uniform white or off-white pellet at the bottom. Reject vials with damaged seals, liquefied cake, discoloration, or visible particulates.
Wipe the rubber stoppers of both vials with an alcohol prep pad and allow to dry.
Draw your calculated volume of bacteriostatic water into the insulin syringe.
Insert the needle into the tesamorelin vial at a 45° angle so the water runs down the inside wall, not directly onto the cake.
Inject slowly. Do not shake the vial. Swirl or gently invert until the cake fully dissolves — typically within 60 seconds. Tesamorelin is more fragile than smaller peptides; vigorous shaking can fragment the molecule.
The solution should appear clear, colorless, and free of particulates.

Storage and stability

Label the vial with reconstitution date, concentration, and lot.
Store reconstituted vials at 2–8°C, protected from light.
Tesamorelin is generally stable for ~14–21 days at refrigerated storage when reconstituted with bacteriostatic water — shorter than many smaller peptides because of its larger molecular structure.
For storage beyond 21 days, aliquot into single-use vials and freeze at −20°C to limit freeze–thaw cycles. Avoid more than 1–2 freeze–thaw cycles per aliquot.
Discard any solution that develops cloudiness, particulates, or color change.

Common reconstitution errors

Shaking the vial. Tesamorelin is structurally sensitive — agitation can denature the peptide. Always swirl or invert.
Direct water impact on the cake. Always run BAC water down the inside wall of the vial, never inject straight onto the lyophilized cake.
Skipping aliquoting for long storage. Repeated freeze–thaw cycles are the main degradation pathway for GHRH-class peptides.
Inconsistent labeling. Always record date, concentration, vial size, and batch number for lab records.

Research use disclaimer

Kalon Research does not provide Certificates of Analysis, purity reports, identity confirmation, or any other quality control documentation with shipments. Tesamorelin is sold strictly for in-vitro and laboratory research use only. Buyer assumes all responsibility for product use, handling, storage, and any consequences thereof.

For Research Use Only. Not for human or animal consumption. Sold strictly for laboratory research purposes. All sales final.