Research tool · Free

Peptide Reconstitution Calculator

Math tool for research labs. Enter your vial mass, bacteriostatic water volume, and target study dose — get the resulting concentration, per-dose draw volume, and U-100 insulin syringe units in one step.

For in-vitro and laboratory research use only. Not dosing advice and not for human or animal administration.

Research tool

Peptide reconstitution math

Calculator for research-use dilution math. Enter vial size, bacteriostatic water volume, and target study dose to see the resulting concentration and draw volume on a U-100 insulin syringe. For laboratory research only. Not dosing advice.

Inputs

Vial size (mg)

Bacteriostatic water (mL)

Common volumes: 1 mL, 2 mL, 3 mL.

Target research dose (mcg)

1 mg = 1000 mcg.

Results

Concentration

5.00 mg/mL

(5000 mcg/mL)

Volume per 250 mcg

0.050 mL

U-100 syringe draw

5.0 units

On a standard U-100 insulin syringe (100 units = 1 mL).

Approximate doses per vial

40.0 doses

Math reference only — for in-vitro and laboratory research use. Not dosing advice and not for human or animal administration. Kalon Research does not represent or warrant product identity, purity, or concentration. Buyer assumes all responsibility.

How to use the calculator

Enter the vial mass in milligrams. This is the total amount of lyophilized peptide in the sealed vial.
Enter the BAC water volume in milliliters. This is the volume of bacteriostatic water you plan to inject into the vial to dissolve the peptide cake.
Enter the target research dose in micrograms. The calculator converts this into the volume to draw from the reconstituted vial.
Read the results: solution concentration (mg/mL and mcg/mL), volume per dose (mL), U-100 syringe units, and approximate doses per vial.

Common reconstitution amounts

Reference table of the resulting concentration for common research vial sizes and bacteriostatic water volumes.

Vial mass

BAC water

Resulting concentration

5 mg
2 mL
2.50 mg/mL (2500 mcg/mL)
10 mg
2 mL
5.00 mg/mL (5000 mcg/mL)
10 mg
3 mL
3.33 mg/mL (3333 mcg/mL)
20 mg
2 mL
10.00 mg/mL (10000 mcg/mL)
30 mg
3 mL
10.00 mg/mL (10000 mcg/mL)
50 mg
5 mL
10.00 mg/mL (10000 mcg/mL)

FAQ

Reconstitution math FAQ

What is peptide reconstitution?

Reconstitution is the laboratory process of dissolving a lyophilized (freeze-dried) peptide powder into a sterile liquid carrier — typically bacteriostatic water — to produce a working solution at a known concentration. The reconstitution math determines how much carrier to add and how much of the resulting solution corresponds to a target study dose.

What does U-100 mean on an insulin syringe?

U-100 refers to an insulin syringe calibrated such that 100 units equal 1 mL. On a U-100 syringe, 50 units = 0.5 mL, 25 units = 0.25 mL, and so on. Research labs use insulin syringes because the unit markings allow precise small-volume draws at the working concentrations typical for research peptides.

How do I convert milligrams (mg) to micrograms (mcg)?

1 mg equals 1000 mcg. If a study uses a 250 mcg target dose and your solution is 5 mg/mL (5000 mcg/mL), the volume per dose is 250 / 5000 = 0.05 mL, which is 5 units on a U-100 insulin syringe.

What if my vial size is not in milligrams?

This calculator assumes vial mass in milligrams (mg). For products supplied in other units, convert to mg before entering — e.g., 1 g = 1000 mg, 100 mcg = 0.1 mg.

Is this calculator dosing advice?

No. This calculator is a math reference for research labs only. It does not recommend, suggest, or imply a dose for human or animal administration. Products from Kalon Research are sold strictly for in-vitro and laboratory research purposes — see the Terms of Service for full terms.

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